Aspergillus quadrilineatus

Aspergillus quadrilineatus Thom & Raper, Mycologia 31: 660. (1939)

Index Fungorum number: IF 275888

Facesoffungi number: FoF 10610

Fungicolous on the fruiting body of Phlebopus spongiosus Pham and Har. Takah. Sexual morph on PDA: Ascomata 190–310 × 160–300 μm (x̅ = 227.6 × 221.6 μm, n = 5), cleistothecial, developing throughout the colony, reddish-brown, globose to subglobose, surrounded by numerous Hülle cells; Hülle cells 12.5–23 × 11.5–22 μm (x̅ = 17.5 × 17.3 μm, n = 30), hyaline to brown, globose to ovoid. Asci 9–11 × 8–11 μm (x̅ = 10.1 × 9.9 μm, n = 15), 8-spored, globose to subglobose. Ascospores 3–4 × 3–4 μm (x̅ = 3.8 × 3.4 μm, n = 40), orange to reddish-brown, globose to subglobose in surface view, spore bodies smooth, lenticular in lateral view, with two plaited equatorial crests about 0.5-1 μm in width paralleled by a secondary narrower pair which are sometimes indistinct, crests are entire, defective or with irregular protuberance. Asexual morph on host: Mycelium powdery mass, white to green; sporulation light green to dark green. PDA: Conidiophores 80–165 × 3–6 μm (x̅ = 122.2 × 4.3 μm, n = 15) macronematous, mononematous, erect, simple, with septate to aseptate stipes, pale brown; Vesicles 9–12 μm (x̅ = 10.6 μm, n = 15) wide, pale brown, globose to subglobose; Metulae 3–4.5 × 1–2 μm (x̅ = 3.7 × 1.5 μm, n = 20) hyaline; Phialides 4.5–6 × 1–2 μm (x̅ = 5.7 × 1.4 μm, n = 20) hyaline, flask-shaped; Conidia 2.5–4 × 2–4 μm (x̅ = 3.2 × 3.3 μm, n = 50) slightly echinulate, globose to subglobose, hyaline to light green (Description from Gajanayake et al. 2021).

Colony characters – on PDA, 25 °C, 14 days: Colonies moderately deep, marginally sulcate; margins slightly irregular; mycelium buff and white, later turns to reddish-brown; texture floccose; sporulation sparse; soluble pigments absent; exudates clear droplets; reverse dark brown.

Material examined – THAILAND, Chiang Rai Province, isolated using the mycelium growing on the fruiting body of Phlebopus spongiosus cultivated in soil, 29 November 2019, Bhavesh Raghoonundon, AJ 044 (inactive dry culture MFLU 21-0261), living cultures MFLUCC 21-0165 and MFLUCC 21-0166.

GenBank accession numbers: MFLUCC 21-0165: ITS: OL615082; tub2: OL625674. MFLUCC 21-0166: ITS: OL615089; tub2: OL625675 (Gajanayake et al. 2021)